A genome is the complete complement of DNA contained in a single organism. One genome may consist of more than one replicating molecule (replicon) such as chromosomes and plasmids. In a finished genome sequence, replicons are one contiguous length of DNA sequence data, or one contig. Genomes that are fragmented into several contigs are considered to be essentially complete when it is a statistical likelihood that greater than 99% of the total nucleotides are represented in the data, and at least 70% of the nucleotides are in contigs at least 20 kilobases in length. For sequences produced by the Sanger method, this requires about 5-fold coverage. For sequences produced by the 454 method, this requires about 10-fold coverage.

Metagenomes? and environmental samples contain DNA from multiple organisms, and these data sets can be stored in the Sprout Database as genomes.

Further Reading

Topic revision: r13 - 16 Jan 2009 - 15:07:46 - TWiki Guest
Notice to NMPDR Users - The NMPDR BRC contract has ended and bacterial data from NMPDR has been transferred to PATRIC (, a new consolidated BRC for all NIAID category A-C priority pathogenic bacteria. NMPDR was a collaboration among researchers from the Computation Institute of the University of Chicago, the Fellowship for Interpretation of Genomes (FIG), Argonne National Laboratory, and the National Center for Supercomputing Applications (NCSA) at the University of Illinois. NMPDR is funded by the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract HHSN266200400042C. Banner images are copyright © Dennis Kunkel.