Using the MG-RAST Metagenome Annotation Server

MG-RAST is designed to rapidly call and annotate the genes from a large set of short DNA sequence reads (icon PMID: 18803844). MG-RAST is based on a modified version of the RAST (Rapid Annotations based on Subsystem Technology) server, which was designed specifically for complete genome sequences. MG-RAST produces automated functional assignments of sequences in the metagenome by comparison with both protein and nucleotide databases. Phylogenetic and functional summaries of the metagenomes are generated, and tools for comparative metagenomics are available. MG-RAST returns an analysis of the genes and subsystems in your data set, as supported by comparative and other forms of evidence.

Users may browse the analysis of public data sets without registering or logging in. If you want to use the server to process your metagenome data set, please register for a free account in order for access to your data to be kept under your control. You may elect to share your private data with others of your choosing, or you may elect to make your data public. Private data will be kept on the server for a minimum of 120 days after completion of processing. Time required for complete processing is largely dependent on the number of jobs in the queue and the size of the data set.

For a complete description of MG-RAST, go to The MG-RAST Blog.

Topic revision: r14 - 23 Jun 2009 - 18:07:18 - Leslie Mc Neil
Notice to NMPDR Users - The NMPDR BRC contract has ended and bacterial data from NMPDR has been transferred to PATRIC (, a new consolidated BRC for all NIAID category A-C priority pathogenic bacteria. NMPDR was a collaboration among researchers from the Computation Institute of the University of Chicago, the Fellowship for Interpretation of Genomes (FIG), Argonne National Laboratory, and the National Center for Supercomputing Applications (NCSA) at the University of Illinois. NMPDR is funded by the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract HHSN266200400042C. Banner images are copyright © Dennis Kunkel.