Using the MG-RAST Metagenome Annotation Server
MG-RAST is designed to rapidly call and annotate the genes from a large set of short DNA sequence reads ( PMID: 18803844
). MG-RAST is based on a modified version of the RAST (Rapid Annotations based on Subsystem Technology) server, which was designed specifically for complete genome sequences. MG-RAST produces automated functional assignments of sequences in the metagenome by comparison with both protein and nucleotide databases. Phylogenetic and functional summaries of the metagenomes are generated, and tools for comparative metagenomics are available. MG-RAST returns an analysis of the genes and subsystems in your data set, as supported by comparative and other forms of evidence.
Users may browse the analysis of public data sets without registering or logging in. If you want to use the server to process your metagenome data set, please register for a free account in order for access to your data to be kept under your control. You may elect to share your private data with others of your choosing, or you may elect to make your data public. Private data will be kept on the server for a minimum of 120 days after completion of processing. Time required for complete processing is largely dependent on the number of jobs in the queue and the size of the data set.
For a complete description of MG-RAST, go to The MG-RAST Blog